TRIzol-Based RNA extraction for detection protocol for SARS-CoV-2 of the coronavirus Disease 2019.
The diagnostic is an important intervention for the management of the 2019 novel coronavirus (SARS-CoV-2). In the present study we developed an optimized protocol for SARS-COV- 2 RNA extraction from the surface of the respiratory mucosa with nasopharyngeal swabs and to compare the sensitivity of RNA extraction methods. Here, RNA extraction was performed using three different procedures (TRIzol, Qiamp, VMT-TRIzol) from nine positive SARS-CoV-2 patients. SARS-CoV-2 was detected by RT-PCR assay using a detection kit for SARS-CoV-2 (Sun Yat-sen University). Comparison of Real-time RT-PCR results, there were no discernible differences in detection rates when comparing the three different extraction procedures. Based on these results, TRIzol as media transport and RNA extraction method for SARS-CoV-2 detection can be a helpful alternative for laboratories facing commercial kit shortages.
Authors: Amirouche Adel, Ait-Ali Djida, Nouri Hamid, Boudrahme-Hannou Lila, Tliba Souhil, Ghidouche Abderrezak, Bitam Idir