Influences of CD8+ Tregs on Peripheral Blood Mononuclear Cells from Allergic Rhinitis Patients.
CD8+ (or CD4+ ) CD25+ fork-head box transcription factor (Foxp3)+ regulatory T cells (CD8+ or CD4+ Tregs) all play a significant role in immune homeostasis and tolerance. However, the role of CD8+ Tregs in allergic rhinitis (AR) have not been clearly elucidated. The present study was aimed to assess the influence of CD8+ Tregs on peripheral blood mononuclear cells (PBMCs) from AR patients.Prospective cross-sectional study.Patients with AR were enrolled. PBMCs were obtained, and CD4+ and CD8+ Tregs were separated from PBMCs and cultured in vitro. We examined percentages of these Tregs in total CD4+ or CD8+ T cells, respectively. After that, we evaluated levels of interleukin (IL)-10 and transforming growth factor (TGF)-β in Tregs cultures. Finally, we administered CD4+ and CD8+ Tregs from AR patients into PBMCs cultures and examined contents of IL-4 and IL-5.The percentages of CD4+ or CD8+ Tregs in the total CD4+ or CD8+ T cells from PBMCs in AR patients were reduced compared to normal subjects. However, IL-10 and TGF-β and their mRNAs were increased in CD4+ and CD8+ Tregs cultures from AR patients, and there were no significant differences in their levels between these two Tregs cultures. IL-4 and IL-5 were increased in AR subjects’ PBMCs compared to normal ones and decreased after the AR CD4+ or CD8+ Tregs administration. However, there were no statistical differences in IL-4 and IL-5 concentrations between these two Tregs treatments.The findings demonstrate that CD8+ Tregs may alleviate inflammatory responses in AR condition.3 Laryngoscope, 2020.