Impact of barrier tissue inflammation and physical activity on joint homeostasis in mice.

Please login or register to bookmark this article
Bookmark this %label%

To investigate whether physical activity interferes with joint homeostasis in the presence of distant inflammation originating at barrier tissues such as skin or gut.Eight-week-old male C57/Bl6 mice were treated with imiquimod cream on a shaved area of the back skin or with dextran sodium sulphate dissolved in the drinking water to induce psoriasis-like skin or inflammatory bowel disease-like gut inflammation. Afterwards, part of the mice was subjected to a 4-week forced running routine (nā€‰=ā€‰10 per group). Severity of cutaneous or intestinal inflammation was assessed clinically, by histology and by quantitative PCR. Knees and paws were analyzed by micro-computed tomography, histology, immunohistochemistry, second-harmonic generation microscopy and quantitative PCR.Local induction of inflammation triggered a systemic response with splenomegaly, loss of bone mass and bone marrow changes. Psoriasis- but not inflammatory bowel disease-like inflammation led to synovial lining layer hyperplasia, an increase in infiltrating CD45+ synovial cells, and suppressed entheseal extracellular matrix gene expression levels. Mechanical loading decreased the amount of F4/80+ synovial macrophages in untreated mice only and led to morphological alterations in the collagen fibers of the enthesis.Systemic inflammation and mechanical loading act independently of each other. The former, originating from distant sites, can trigger mild synovial inflammation in mice, a propensity that may also impact the development of arthritis in patients; the latter has no impact on the severity of systemic inflammation, but independently affects joint homeostasis.

View the full article @ Rheumatology (Oxford, England)
Get PDF with LibKey

Authors: Margot Van Mechelen, Tobie Martens, Pieter Vanden Berghe, Rik Lories, Giulia R Gulino