Beclin1 in Psoriasis: An Immunohistochemical Study.
Abnormal autophagy is now established to be associated with pathogenesis of some skin disorders. Beclin1 plays a central role in the machinery of the autophagy.to assess the expression of “Beclin1” in psoriasis through its immunohistochemical study in lesional and perilesional skin in psoriasis cases.This case-control study was carried out on a total number of 40 subjects, 20 patients with chronic plaque psoriasis and 20 age and sex matched apparently healthy subjects as a control group. Skin biopsies were taken from lesional, perilesional skin of psoriasis patients and normal skin from healthy controls for immunohistochemical evaluation of Beclin1 expression’.Epidermal Beclin1 expression was positive in the three studied groups. There was a significant difference between the 3 studied groups regarding epidermal topographic distribution of Beclin1, epidermal intensity of staining, Beclin1 H score and H score category (P >0.01 for all). Significant differences were found between the 3 studied groups regarding skin adnexal Beclin1 H score and H score category (P >0.01 for both). Regarding inflammatory infiltrate Beclin1 expression, significant differences were found between lesional and perilesional skin regarding expression status and H score (P >0.01 for both) and significant differences regarding intensity of staining, and H score category (P= 0.014 and 0.029 respectively).The increased expression of Beclin1 in psoriasis, lesional and perilesional, reflecting increased autophagy which could be a consequence of the rapid keratinocyte proliferation that must also ramp up all the cellular processes including autophagy. The cellular localization of Beclin1 was nucleocytoplasmic in psoriasis while cytoplasmic only in normal skin of controls which needs interpretation in further study.